IOBC-WPRS Shop

View basket

IOBC-WPRS Bulletin Vol. 133, 2018

 

IOBC-WPRS Bulletin Vol. 133, 2018

Working group "Biological and Integrated Control of Plant Pathogens"
Preceedings of the XV Meeting "Biocontrol products: From lab testing to product development" at Lleida (Catalonia, Spain), April 23-26, 2018
Edited by: Jürgen Köhl, Marc Bardin and Philippe Nicot
ISBN 978-92-9067-318-7 [XVI + 215 pp]

 

25.00 €

 

 

 

 

Add this product to your basket

Articles

Pages

Price

Cart

New concepts in the identification and practical application of biocontrol agents against plant pathogens
Ilaria Pertot, Michele Perazzolli, Gerardo Puopolo

Extended abstract

2-3

0.00 €

 

Efficient biocontrol of the phytopathogen Ralstonia solanacearum by novel lytic bacteriophages in irrigation water, in planta and after mass production
Belén Álvarez, María M. López, Elena G. Biosca

Abstract: The phytopathogen Ralstonia solanacearum produces bacterial wilt, one of the most damaging plant diseases worldwide. A number of control methods have been applied in the field with either environmental impact and/or uncertain success, mainly due to the high pathogenic potential of the bacterium and its long-term persistence in the environment. Biological control strategies have the advantage of being able to be easily integrated in a sustainable agricultural system. In this sense, lytic bacteriophages (phages) can destroy their target specifically without impact on the ecological balance. Ralstonia solanacearum lytic phages were isolated and their biocontrol ability tested in environmental water, and in planta. Results revealed either reductions in R. solanacearum populations in water or bacterial wilt incidence in the host, with absence of symptoms in many of the plants. Phages were mass produced in bioreactors and their biocontrol ability tested in the same way with similar results, indicating a good potential of the newly isolated phages for bacterial wilt biocontrol in conditions mimicking those of natural settings.

4-8

5.00 €

 

Novel isolates of Ralstonia and Mitsuaria species as biocontrol agents for controlling tomato bacterial wilt
Malek Marian, Tomoki Nishioka, Hiroyuki Koyama, Haruhisa Suga, Masafumi Shimizu

Extended abstract

9-10

0.00 €

 

Suppressive effect of aquaponic water on the development of root rot caused by Pythium aphanidermatum in lettuce
Gilles Stouvenakers, Sébastien Massart, Haïssam M. Jijakli

Abstract only

11-12

0.00 €

 

An in planta method for the screening of Plant Growth Promoting Rhizobacteria to control plant pests and pathogens
Trimurti Habazar, Yulmira Yanti

Abstract: Plant pests and diseases are among the most important biotic agents causing serious losses and damages to agricultural production. A number of different strategies are currently being employed to manage and to control them, especially using pesticides. As we know, the synthetic pesticides have a negative effect on environment and health. This leads to the enormous interest in finding effective non chemical alternatives to protect plants against pests and diseases, such as biocontrol agents (BCA). One group of important BCA is the Plant Growth Promoting Rhizobacteria (PGPR). This paper explains the effective method to find PGPR strains, which have the ability to control plant pest and diseases, and also to increase growth and yield of plant. This method is based on in planta selection of PGPR from healthy plants in pest or disease endemic areas. This approach focuses on general forms of disease suppression, especially indirect mechanisms, such as induced systemic resistance. We characterized only the best PGPR strains which have several mechanisms, such as to control plant pest and disease and to promote growth and to increase yield of plants. This technique has the possibility to find the new, easy and cheap biocontrol organisms. Our previous study showed that PGPR strains from the rhizosphere of healthy plants could control plant pests and pathogens and also increase plant growth and yield, such as 10 PGPR strains from rhizosphere of healthy ginger to control Ralstonia solanacearum race 4; 13 PGPR strains from rhizosphere of healthy chili to control bacterial wilt disease; 3 indigenous PGPR strains and 2 non-indigenous PGPR’s strains to control the armyworm on onion; 12 PGPR strains from rhizosphere of healthy onion to control Xanthomonas leaf blight disease. The best PGPR strains from onion’s rhizosphere were identified as Pantoea agglomerans, Enterobacter cloacae, Stenotrophomonas sp., Serratia marcescens, five Bacillus thuringiensis strains, B. cereus, B. weihenstephanensis and Bacillus sp. We have obtained the new information, that B. thuringiensis strains could control Xanthomonas leaf blight on onion. As we know, these bacteria commonly act as entomopathogen to control insect pest.

13-17

5.00 €

 

Botrytis bunch rot in vineyards: still a challenge for commercial biological control products and novel microbial antagonists
Carlos Calvo-Garrido, Neus Teixidó, Josep Usall, Rosario Torres, Nicolas Aveline, Marc Fermaud

Extended abstract

18-19

0.00 €

 

Development of a new biological control product for powdery mildew control in cereals
Jürgen Köhl, Carin Lombaers-van der Plas, Trudy van den Bosch, Helen Goossen-van de Geijn, Lia Groenenboom-de Haas, Huub Schepers, Ulrike Hilscher, Mariann Wikström, Carolien Zijlstra

Abstract: Powdery mildew caused by Blumeria graminis is a major fungal disease in cereal crops. The objective of our research was to select fungal antagonists for control of Blumeria graminis f.sp. tritici in wheat. More than 1200 fungal isolates were obtained from powdery mildew pustules on leaves collected in The Netherlands, Germany and Sweden. Isolates were tested for spore production, cold tolerance, UVb resistance, drought tolerance and safety. The effect of selected isolates on powdery mildew sporulation on winter wheat seedlings was quantified. Superior isolates were subsequently applied on spring wheat under open field conditions. Two isolates significantly slowed down the progression of powdery mildew epidemic in the spring wheat crop.

20-22

5.00 €

 

Characterization of the biocontrol of Phytophthora infestans and Alternaria solani by bacteria isolated from Belgian agroecosystems
Gil Colau, Florent Licciardi, Simon Caulier, Annika Gillis, Jacques Mahillon, Claude Bragard, Anne Legrève

Abstract only

23

0.00 €

 

Screening of efficient bacterial control agents against two fungal pathogens, Phaeomoniella chlamydospora and Neofusicoccum parvum, involved in grapevine trunk diseases
Rana Haidar, Jean Roudet, Alain Deschamps, Patrice Rey and Marc Fermaud

Abstract only

24

0.00 €

 

Antifungal activity of Ecklonia sp. and Jania sp. polysaccharides against Botrytis cinerea
H. Righini, A. Martel-Quintana, Y. García-Fernández, J. L. Gómez-Pinchetti, R. Roberti, E. Baraldi

Extended abstract

25-26

0.00 €

 

Effect of microscopic fungi on larval mortality of the root-knot nematodes Meloidogyne incognita and Meloidogyne javanica
Varvara Migunova, Nicola Sasanelli, Alexander Kurakov

Abstract: Fungal antagonists of plant parasitic nematodes producing toxic metabolites that kill nematodes were found in an in vitro test. The mortality of second stage juveniles of root knot nematodes was assessed by hatching tests. Nine strains of soil microscopic fungi of genera Arthrobotrys, Trichoderma, Purpurocillium and Clonostachys were tested for their nematicidal activity against second stage juveniles of Meloidogyne incognita and Meloidogyne javanica. Mortality of larvae varied from 92.7 to 100% for all strains after 48 hours exposure time. Arthrobotrys, Clonostachys, Purpurocillium lilacinum and Trichoderma strains showed different nematicidal effects according to the nematode species. Only Trichoderma sp. KAV1 and C. rosea KAV2 showed 100% mortality for both nematode species.

27-31

5.00 €

 

Characteristics of biocontrol agents of Diplodia seriata, one of the pathogens causing “Botryosphaeria dieback” on vine plants in Chile
Javiera Molina, Mauricio Ramírez, Jaime Montealegre and Luz M. Pérez

Abstract: The fungal development and conidial germination of Trizian 1 and of Closea 1, two biocontrol agents of Diplodia seriata, was not affected by pH values ranging from 4.5 to 7.5 or by temperatures ranging from 5 °C to 37 °C or by several agrochemicals, used to prevent trunk wood vine diseases other than those caused by Botryosphaeria. These characteristics are important for the development of a formulation containing these biocontrol agents (BCAs), where the development and production parameters should take them into account.

32-35

5.00 €

 

Screening of bacteria and fungi for activity against soil-borne Fusarium infections of maize seedlings
Tobias Pfeiffer, Eckhard Koch, Jannika Drechsel, Tim Birr

Extended abstract

36-37

0.00 €

 

Identification and biocontrol of antagonistic yeasts against Botrytis cinerea on different hosts
Jessica Marisol Martínez Martínez, Ramón Álvar Martínez Peniche, Lourdes Soto Muñoz

Abstract only

38

0.00 €

 

Virulence enhancement of weed biocontrol agents by elevating their amino acid excretion
David C. Sands, Henry S. Nzioki, Florence Oyosi, Claire S. Baker, Edward Dratz

Abstract only

39

0.00 €

 

Biological control of hairy roots disease in greenhouse vegetables
Marta A. Stremińska, Ineke Stijger

Abstract only

40

0.00 €

 

Impact of Sclerotinia sclerotiorum control by Trichoderma strains on soybean yield
Cleusa Maria Mantovanello Lucon, Ricardo Harakava

Abstract only

41-42

0.00 €

 

Aromatic plant interrows to control grapevine moth Lobesia botrana populations in vineyards
Ana M. Díez-Navajas, J. Frutos

Abstract only

43

0.00 €

 

Antifungal activity of essential oil from Coriandrum sativum on seed-borne pathogens
Alma Valiuškaitė, Edita Dambrauskienė, Lina Šernaitė, Rasa Karklelienė, Neringa Rasiukevičiūtė

Abstract only

44

0.00 €

 

Antifungal activities of the leaf and sap extracts of two endemic Aloe species against seed borne fungal pathogens (Aspergillus species) of groundnut (Arachis hypogaea L.)
Dinkayehu Alamnie, Ameha Kebede, Meseret Chimdessa

Abstract only

45

0.00 €

 

Evaluation of leaf extracts of four plant species against rice blast pathogen (Magnaporthe oryzae)
M. O. Adebola, O. B. Ayeni, M. B. Aremu

Abstract only

46

0.00 €

 

Evaluating the biocontrol efficiency of six medicinal plant extracts against watermelon bacteria blotch disease caused by Acidovorax citrulli
M. O. Adebola, H. M. Mohammud, I. M. Bello, O. A. Kalesanwo

Abstract only

47

0.00 €

 

Essential oils from Thymus vulgaris and Coriandrum sativum for the control of Fusarium graminearum
Neringa Rasiukevičiūtė, Skaidrė Supronienė, Alma Valiuškaitė, Armina Morkeliūnė, Edita Dambrauskienė

Abstract only

48

0.00 €

 

Aromatic plants activity on Alternaria spp. pathogen
Alma Valiuškaitė, Edita Dambrauskienė, Neringa Rasiukevičiūtė

Abstract only

49

0.00 €

 

Biocontrol agents: development and implementation aspects
Antonieta De Cal, Inmaculada Larena, Belén Guijarro, Paloma Melgarejo

Abstract only

51

0.00 €

 

Efficacy and effect of novel film-forming formulations of the biocontrol agent Candida sake CPA-1 applied at field conditions in organic wine grapes
Anna Carbó, Rosario Torres, Josep Usall, Amparo Chiralt, Anna Marín, Neus Teixidó

Abstract only

52

0.00 €

 

Controlling fire blight (Erwinia amylovora) infection of apple and pear flowers using biocontrol organisms dispersed by pollinating insects
Bart Vanhoutte, Bart Cottyn, Cinzia Van Malderghem, Jolien Smessaert, Matti Pisman, Hilde Schoofs, Guy Smagghe, Martine Maes, Wannes Keulemans, Olivier Honnay, Dany Bylemans, Serge Remy

Extended abstract

53-54

0.00 €

 

The inhibitory activity of the biocontrol agent Lysobacter capsici AZ78 is negatively modulated by Bacillus spp. isolated from grapevine leaves
Francesca Brescia, Lisa Ioriatti, Oscar Giovannini, Gerardo Puopolo, Ilaria Pertot

Extended abstract

55-56

0.00 €

 

Formulation development of biological products from lab to commercial scale
Pankaj Pathak

Abstract only

57

0.00 €

 

Bacillus subtilis MBI 600: A new potential biocontrol agent as an alternative solution against Botrytis cinerea in cucumber plants
Anastasios Samaras, A. Chaliotis, G. D. Amoutzias, and George S. Karaoglanidis

Extended abstract

58-59

0.00 €

 

Development of biological control products through vertical integration and global collaboration
Molly Cadle-Davidson, Ronél Hendriks

Abstract: Maintenance of diversity of thought and independent research centers, whether academic, government or corporate, is essential to continued success in developing microbe-based agricultural tools. ABM has a unique business model that enables it to develop efficacious biological control/stimulant products on an accelerated timeline despite its small size. A case study, including strategy and supporting data, of a Trichoderma atroviride-based nematicide is presented from discovery to release of an EPA registered product. Key factors in maintaining an independent, research-driven company focused on delivering biocontrol products to growers are discussed.

60-64

5.00 €

 

Strategy for the discovery, development and delivery of biopesticides: A case study
Alba Marina Cotes, Xavier Fargetton

Abstract only

65

0.00 €

 

Validation of a biological control strategy against brown rot in different European stone fruit producing areas
Neus Teixidó, Antonieta De Cal, Carla Casals, Rosario Torres, Belén Guijarro, Víctor Perdrix, Ulrike Hilscher, Edith Ladurner, Tom Smets, Josep Usall

Abstract only

66-67

0.00 €

 

Lab and field tests with Metschnikowia pulcherrima as a possible biocontrol agent against storage diseases of apple
Sarah Perren, Florian Freimoser

Abstract: Storage diseases cause significant losses in pome fruit production and are caused by a plethora of different fungal pathogens that are already present on the fruit at the time of harvest. Due to the diversity of fungal diseases and the extremely high requirements of consumers and retailers regarding the fruit quality, integrated fruit production requires regular interventions with plant protection agents and strategies. Pome fruit production thus requires a large input of plant protection agents (Spycher & Daniel, 2013) and consequently has ample potential for improving plant protection measures and developing low-risk applications. In addition, authorities in Switzerland and several other European countries demand a reduction of health and environmental risks caused by plant protection agents of at least 50% (Bundesrat, 2017). To achieve this goal and maintain the production of high-quality apples, fruit growers need information and advice on designing and operating innovative, sustainable and reliable production systems (Gölles et al., 2015). Therefore, as an alternative to chemical-synthetic fungicides, we are assessing the potential of naturally occurring yeasts as antagonists of apple storage diseases.
Yeast-like fungi have been identified as effective antagonists of fungal plant pathogens, but only few yeast species were developed as commercial plant protection products so far. It is our aim to isolate and identify antagonistic yeasts, to characterise the modes of antagonism of the strongest antagonists against plant pathogens, and to assess their potential as plant protection agents against apple storage diseases in the field. Ultimately, it is our goal to establish sustainable apple production systems that require substantially less chemical-synthetic pesticides and result in significantly lower residues on apples.

68-72

5.00 €

 

Helping farmers in timing the application of biocontrol agents in viticulture
E. González-Domínguez, S. Legler, G. Fedele, M. Si Ammour, T. Caffi, V. Rossi

Abstract: Directive 128/2009/EC on the Sustainable Use of Pesticides encourages EU Member States to promote low pesticide-input pest control by the implementation of tools for pest monitoring and decision making, and by giving priority to non-chemical methods, including the use of biological control agents (BCAs). Although the intensive research developed in the last decades the practical use of BCAs is still challenging. Biocontrol of plant diseases involves complex interactions between two living organisms (the pathogen and the BCA), the plant and the environment. To achieve effective disease management is then crucial having knowledge on: (i) the life cycle of both, the pathogen and the BCA; (ii) the mode of action of the BCA against the pathogen; and (iii) how the two are influenced by the environment. Mathematical models for pathogen and BCAs could help understanding these relationships and then contributing to optimisation of the BCA applications. In this work, two examples are presented on how the use of BCAs can be improved by using mathematical modelling: Ampelomyces spp. hyperparasites against Erysiphe necator (the grape powdery mildew fungus), and BCAs against Botrytis cinerea (causing Botrytis bunch rot in grapes).

73-75

5.00 €

 

Control of fruit and leaf diseases of olive with a pomegranate peel extract
Leonardo Schena, S. Pangallo, G. E. Agosteo, M. G. Li Destri Nicosia, F. V. Romeo, P. Rapisarda, A. Abdelfattah, S. Mosca, S. Scibetta, G. Magnano di San Lio, S. O. Cacciola

Extended abstract

76-77

0.00 €

 

Soaking seed with chitosan promotes wound healing of harvested potato tubers
Yang Bi, Jingrong Zhang, Huali Xue, Hong Jiang, Xiangzhen Ge, Yan Zhu

Abstract only

78

0.00 €

 

New advances in the formulation of the biopreservative strain CPA-7 of Pseudomonas graminis
Núria Baró-Montel, Cristina Solsona, Inmaculada Viñas, Rosario Torres, Josep Usall, Neus Teixidó

Abstract: The biopreservative strain CPA-7 of Pseudomonas graminis has shown its potential for inhibiting growth of foodborne pathogens such as Salmonella, Listeria monocytogenes and Escherichia coli O157:H7 in fresh-cut fruit. However, little success rate of biocontrol agents based products are available in the market mainly due to the maintenance of product shelf-life and efficacy. Therefore, the aim of this study was to obtain a P. graminis CPA-7-based product that maintained the viability over time. To achieve this objective, CPA-7 fresh cells grown on an optimized soy protein based medium were harvested by centrifugation, concentrated and formulated using two different techniques: a) liquid formulation (CPA-7 cells suspended in liquid solutions with different protective substances) and b) freeze-drying formulation (CPA-7 fresh cells amended with different protective substances and then freeze-dried). All CPA-7-based products were maintained under refrigeration conditions (4 °C) and the effect of protectants on cell viability and storage stability was determined. According to results obtained, liquid formulation could not be considered suitable because some shortcomings as limited shelf-life but freeze-drying has allowed to improve the formulation of CPA-7. The stable freeze-dried products obtained combined with disinfectant methods could be an additional hurdle to ensure the microbiological safety of minimally processed fruit as well as a promising tool to cover consumer demand for additive-free products.

79-85

5.00 €

 

Impact of UV-C radiation combined with biocontrol agents on the susceptibility of strawberry plants to Botrytis cinerea
Marine Forges, Florence Charles, Michel Pascal, Jawad Aarrouf and Marc Bardin

Abstract: In order to enhance biocontrol efficacy against plant diseases, the combination of different control methods together with a given biocontrol agent can be achieved. In this study we have tested the effect of combining UV-C radiation with the application of biocontrol agents (Bacillus subtillis and Gliocladium catenulatum based products) against Botrytis cinerea on strawberry. We hypothesize that UV-C radiation applied on plants previously to biocontrol treatment, in addition to its induced resistance effect, will somehow disinfect leaves thus limiting the competition of inoculated biocontrol agents with the phyllosphere microorganisms and then promoting its development and its efficacy. We have shown that, despite the confirmed germicidal effect of UV-C radiation, this treatment applied on plants before treatment with biocontrol agent has no effect on the level of efficacy of the biocontrol agents or even it tends to lower their efficacy. Work is in progress to understand mechanisms involved and to evaluate the effect of this combination of treatments on other plant species.

86-90

5.00 €

 

Biological control of yellow rust in spring wheat in Latvia 2017
Liga Feodorova-Fedotova, J. Landorfs, O. Treikale

Abstract only

91

0.00 €

 

Evaluation of the biofungicides Timorex Gold®, STK-73 and Serenade ASO® on the control of gray mold (Botrytis cinerea) in blueberries cv. Jewell and cv. Star. Chillan, Bio-Bio Region, Chile
Juan C. Arroyo, A. France, G. Sir

Abstract only

92

0.00 €

 

Seedling treatments with biological pesticides and biostimulants against Fusarium basal rot on onion
Emmi Kuivainen, Minna Haapalainen, and AskoHannukkala

Abstract only

93

0.00 €

 

Elucidation of boundaries for the effective use of biological control organisms against wound pathogens causing fungal storage diseases in apples
Tanja Vanwalleghem, Charity Christian Andoh, Wendy Van Hemelrijck, Dany Bylemans

Abstract only

94

0.00 €

 

Strategies for organic control of tomato bacterial spot
G. Bischetti, G. Giovanale, A. Mazzaglia, E. Fortunati, G. M. Balestra

Abstract: In this research the possibility has been verified to reduce current copper salt field doses (hydroxide) suggested to control Xanthomonas axonopodis pv. vesicatoria (Xav) (Doidge) Dye, the causal agent of tomato bacterial spot. In vitro and in planta tests were performed to evaluate and compare different botanical extracts and an essential oil (coumarin, gallic acid, carvacrol), alone or in combination, in respect to copper hydroxide. Plant extracts in combination with a reduced amount of copper salts, have been found to be as effective as the copper dosages recommended to date for control of Xav, proposing new biological strategies for the control of this dangerous pathogen.

95-97

5.00 €

 

Environment-friendly postharvest technologies reducing strawberry fruit contamination
Neringa Rasiukevičiūtė, Alma Valiuškaitė, Nobertas Uselis, Živilė Lukšienė

Abstract only

98-99

0.00 €

 

Diversity in the susceptibility of Botrytis cinerea strains to the biological control agent Pseudomonas helmanticensis
Yousra Bouaoud, Claire Troulet, Kamel Aissat, Marc Bardin

Abstract: The strain Pseudomonas helmanticensis CT22 isolated from tomato stem in a tomato greenhouse in Bejaia (Algeria) has shown a good protective efficacy against Botrytis cinerea in controlled conditions but its effect against various strains of B. cinerea was not assessed. In this study, we evaluated the diversity in the sensitivity of sixty two strains of B. cinerea strains to P. helmanticensis CT22 both in vitro and on tomato. In vitro effect of the biocontrol agent is variable according to the strain of B. cinerea, both in dual culture assay or in volatile assay. Due to the very high level of efficacy against all tested strains of B. cinerea on detached tomato stem when P. helmanticensis CT22 was applied at 107 CFU/ml, the dose of application was reduced 10-fold at 106 CFU/ml. In this condition, the protective efficacy of the biocontrol agent is significantly influenced by B. cinerea strains with protection levels ranging from 24% to 100%. These results suggest a diversity in the sensibility of the different strains of B. cinerea to the biological control agent and emphasize the importance to consider several strains of a given plant pathogen species when screening a biocontrol agent.

100-104

5.00 €

 

Medium optimization for enhancing antifungal HSAF production by Lysobacter enzymogenes OH11
Fengquan Liu, Bao Tang

Abstract only

105

0.00 €

 

Antifungal activity of Bacillus amyloliquefaciens BUZ-14 and B. methylotrophicus CECT 8661 against the grey rot in vineyards
L. Toral, H. Calvo, I. Mendiara, V. Béjar, I. Sampedro, D. Blanco, M. E. Venturini

Extended abstract

106-107

0.00 €

 

From the mass production and formulation to the fate in the environment of Lysobacter capsici AZ78, a biological control agent of Plasmopara viticola
Gerardo Puopolo, Guillem Segarra, Elena Porcel-Rodriguez, Oscar Giovannini, Ilaria Pertot

Abstract: The antagonistic bacterial strain Lysobacter capsici AZ78 was recently selected for its relevant efficacy in controlling Plasmopara viticola under greenhouse conditions. Since little is known about how Lysobacter spp. may respond to desiccation, rain wash-off, and UV light, the aim of this work was to design formulations, which can effectively protect L. capsici AZ78 cells by these environmental stressors when applied in the vineyards. Firstly, fermentation conditions were optimized to achieve a harvest of 1010 L. capsici AZ78 cells/ml after 24 h and the shelf-life at 4 °C indicated only a decrease of an order of magnitude after one year. Polyethyleneglycol, corn steep liquor and lignosulfonate were subsequently selected for the protection of L. capsici cells against desiccation, wash-off and UV light and were combined to implement a liquid formulation. The efficacy of L. capsici AZ78 formulated cells against P. viticola on grapevine under field conditions was higher than the unformulated ones. Finally, the fate in the environment of L. capsici AZ78 cells was assessed through a strain specific qPCR protocol. Results showed that the negative effect played by solar radiation, rainfall, unfavourable temperature and relative humidity on the unformulated AZ78 cells was mitigated by the formulation. The procedure followed in this work allowed to produce a first prototype formulation of L. capsici AZ78, that made this biocontrol agent more efficient in persisting on grapevine leaves and, consequently, controlling P. viticola under field conditions.

108-110

5.00 €

 

Application of isothermal microcalorimetry for rapid quantification of bacterial and fungal biocontrol agents
J. Nykyri, A. M. Herrmann, S. Håkansson

Abstract only

111-112

0.00 €

 

Utilizing metagenomics to improve efficacy of postharvest biocontrol agents
Samir Droby, Michael Wisniewski

Abstract only

114

0.00 €

 

Designing a dual RNA-Seq approach to dissect the mode of action of biocontrol bacteria against phytopathogenic eukaryotes
Gerardo Puopolo, Selena Tomada, Paolo Sonego, Marco Moretto, Kristof Engelen, Ilaria Pertot, Michele Perazzolli

Abstract: Understanding the mode of action of biocontrol agents plays a key role in the development of novel and effective biofungicides. We propose a transcriptome approach based on dual RNA-Seq to understand, at molecular level, the interaction between biocontrol bacteria and their targeted phytopathogenic fungi and/or oomycetes. This approach was validated on the interaction between the biocontrol bacterium Lysobacter capsici AZ78 and the phytopathogenic oomycete Phytophthora infestans. The RNA-Seq transcriptional profile showed that L. capsici AZ78 cells spread on the mycelium of P. infestans by up-regulating genes involved in biofilm disaggregation and the biogenesis of type 4 pilus. Lysobacter capsici AZ78’s genes involved in antibiotic biosynthesis were up-regulated and they may be linked to the cell death of P. infestans, where putative apoptotic processes were activated and resulted in the down-regulation of primary metabolic pathways. Lysobacter capsici AZ78 cells fed of dead P. infestans mycelium by up-regulating genes encoding lytic enzymes involved in the attack of the main components of oomycete cell walls. Overall, the dual RNA-Seq approach clarified the mode of action of L. capsici AZ78, which is associated with parasitism and predatory activities that cause the death of P. infestans.

115-118

5.00 €

 

Mechanistic characterization of the antagonistic interaction between Collimonas fungivorans Ter331 and Aspergillus niger N402
Sandra Mosquera, Johan Leveau, Ioannis Stergiopoulos

Extended abstract

119-120

0.00 €

 

Differential interaction of dark septate endophytes and fungal pathogens in vitro and in planta
Wael Yakti, Gábor M. Kovács, Philipp Franken

Abstract only

121

0.00 €

 

Elucidating the mechanisms of action of the non-pathogenic strain of Fusarium oxysporum FO12 against Verticillium wilt of olive
A. Mulero-Aparicio, T. Cernava, F. J. López-Escudero, G. Berg, A. Trapero

Extended abstract

122-123

0.00 €

 

Is the development of resistance to biological control among plant pathogens possible?
Marc Bardin, Philippe C. Nicot

Abstract: Although many plant pathogens are known for their capacity to develop resistance to classical control methods, only few studies have explored their ability to overcome the effect of biocontrol agents. However, these studies highlight differences in the sensitivity of various isolates of plant pathogens to biocontrol agents or to compounds that are produced or synthesized as the result of their interaction with the host-plant. This observed diversity may contribute to the inconsistent efficacy of biocontrol agents sometimes observed in the field. Moreover the selection pressure exerted by an increase in the use of biocontrol agents may cause the emergence of new resistant phenotypes from these less sensitive isolates. This review highlights current knowledge concerning erosion of biocontrol against plant pathogens and its possible consequences for field efficacy.

124-126

5.00 €

 

Role of the Moroccan Streptomyces in the induction of system defense of pea against Aphanomyces euteiches
Brahim Oubaha, Ahmed Nafis, Fanny Louviot, Aboubakr Moradi, Felix Mauch, Mustapha Barakate

Abstract only

127

0.00 €

 

Colonization of the phylloplane of banana plants by Bacillus subtilis EA-CB0015
Tatiana Z. Cuellar-Gaviria, Lina María González-Jaramillo, Javier Correa-Álvarez, Valeska Villegas-Escobar

Abstract only

128-129

0.00 €

 

Role of Burkholderia-derived pyochelin in the Fusarium wilt suppression of Allium-cultivated soil
Tomoki Nishioka, Chihiro Tanaka, Malek Marian, Yasuhiro Igarashi, Haruhisa Suga, Masafumi Shimizu

Abstract only

130-131

0.00 €

 

Different modes of action of the two Aureobasidium pullulans L1 and L8 strains biological control agents
Alessandra Di Francesco, Marta Mari, Elena Baraldi

Extended abstract

132-133

0.00 €

 

The role of water-soluble and volatile secondary metabolites of Pseudomonas donghuensis P482 in antimicrobial activity
Sylwia Jafra, Adam Ossowicki, Magdalena Jabłońska, Marta Matuszewska, Paolina Garbeva

Abstract only

134-135

0.00 €

 

Histological studies on the establishment of putative endophytic fungi in leaf tissue
Eckhard Koch, Petra Zink, Cornelia I. Ullrich, Regina G. Kleespies

Abstract only

136

0.00 €

 

Interaction of dark septate endophytes with arbuscular mycorrhizal fungus Rhizoglomus irregulare
Wael Yakti, Gábor M. Kovács, Philipp Franken

Abstract: Dark septate endophytes (DSEs) are ascomycetous root colonizer fungi distributed widely, and commonly found in environments with strong abiotic stress. DSEs are regarded as potential plant promotion agents and were shown to help plants against fungal diseases. Besides being ubiquitous, the ecological function of DSEs is not well understood. Although they co-occur in the root with beneficial plant microbes, our knowledge e.g. about the interaction between DSEs and other common fungi, such as the arbuscular mycorrhizal fungi (AMF) is poorly understood. Considering that antagonism with AMF is a negative prosperity of inocula developed for agricultural use, it is important to test whether fungi, that show potential as bio-fertilizers, would have negative effect on plant colonization by other beneficial microbes. In the current study, we performed a greenhouse experiment using tomato plants to study the interaction between two model DSEs Periconia macrospinosa and Cadophora sp., and the AM fungus Rhizoglomus irregulare. The effect of inoculation of AMF on plants was evaluated with and without the DSEs, and the colonization rate of AMF between the treatments was assessed using microscopy and qRT-PCR assays. The results show that the DSEs did not influence the effects of the AMF Rhizoglomus irregulare on the host plants.

137-141

5.00 €

 

Genomic sequencing of Pasteuria penetrans, an obligate parasite of Meloidogyne spp.
Jamie Orr, Peter Cock, Keith Davies, Vivian Blok

Abstract only

142-143

0.00 €

 

Effects of elicitors on trichothecene accumulation and Tri genes expression in potato tubers inoculated with Fusarium sulphureum
Huali Xue, Yang Bi, Yuanyuan Zong, Lumei Pu, Haitao Long, Minan Nan

Abstract only

144

0.00 €

 

Mechanism research of transcriptional regulator LeClp in the biosynthesis of WAP-8294A2 in Lysobacter enzymogenes
Huiyong Xu, Yangyang Zhao, Guoliang Qian, Fengquan Liu

Abstract only

145

0.00 €

 

Antifungal activity and action mode of HSAF from Lysobacter enzymogenes against Fusarium graminearum
Yangyang Zhao, Chao Cheng, Fengquan Liu

Abstract only

146

0.00 €

 

Antibacterial mechanism of luteolin-7-O-glucoside from artichoke extracts against bacterial plant pathogens
Pedro Laborda, Jun Ling, Xian Chen, Fengquan Liu

Abstract only

147

0.00 €

 

Elucidating the mode of action of licorice as a new biocontrol solution against oomycetes and phytopathogenic bacteria
Sophie Hermann, Adam Schikora, Ina Kleeberg, Andreas Scherf, Marc Orlik and Annegret Schmitt

Extended abstract

148-149

0.00 €

 

Volatile organic compounds emitted upon infection of barley roots by fungal pathogens: antifungal effects and mode of action
Caroline De Clerck, Marie Fiers, Lucie Jallais, Olivier Parisi, Marie-Laure Fauconnier, Sébastien Massart, Jijakli M. Haissam

Abstract only

150

0.00 €

 

Temperature affects Bacillus antimicrobial compounds production but not the biocontrol of common bean diseases
Andrea B. Moura, J. P. Fasolin, M. Sangiogo, I. T. Souza-Junior, B. Rohrig, R. Moccellin

Extended abstract

151-152

0.00 €

 

Build-up of a Bacillus strain collection for strains with high potential as plant biocontrol agents based on a promising biocontrol candidate
Gajender Aleti, Angela Sessitsch, Stéphane Compant, Nikolaus Pfaffenbichler, Friederike Trognitz, Günter Brader

Abstract only

153

0.00 €

 

Endophytic Bacillus screening and identifications as growth promotor and biocontrol agent against Colletotrichum capsici on chili
Yulmira Yanti, Reflin, Warnita, Chainur Rahman Nasution, Nuzuli Rahmadani

Abstract only

154

0.00 €

 

Bacillus atrophaeus strain L193 surfactants for the biocontrol of aphids in cereal crops
M. Rodríguez, L. Toral, A. Marín, M. Torres, V. Béjar, M. Campos, I. Sampedro

Extended abstract

155-156

0.00 €

 

Production of microbial biological control agents
Ralf-Udo Ehlers

Abstract only

158

0.00 €

 

Trichoderma asperellum strain t34: From the laboratory-patent to the registration-market
Maria Isabel Trillas, Eva Casanova, Elena Fernández, Guillem Segarra

Abstract: At this moment (Regulation (EC) 1107/2009 and Directive 2009/128/EC), there are obvious reasons for the development of antagonist microorganisms to incorporate in integrated pests and diseases management programs. The identification of strain T34 of Trichoderma asperellum as a biological control was carried as part of research projects at the University of Barcelona (1998). The strain was patented in 2002 and it is currently being developed by Biocontrol Technologies, according to the EU directives on plant protection products. Different from other spin-offs, we are involved in all phases of development (from discovery to marketing), always in strong relationship with other universities, research centers and companies, in different countries. Our aim is to conduct basic research, but also to offer commercial biological control solutions to growers.

159-163

5.00 €

 

Development of a new biocontrol product (AUREO® Gold) for control of Pseudomonas syringae pv. actinidiae in kiwifruit
Stephen Hoyte, Philip Elmer, Frank Parry, Janet Phipps, Mike Spiers, Joe Taylor, Annette Ah Chee, Judith Rees, Sean Marshall, David Wright, Kirstin Wurms, Jennifer Parry, Kirsty Lyall, Tony Reglinski,, Nicola Mauchline, Huub de Jong, Claire Hall, Kirsty Boyd-Wilson, Ron van Toor, Joel Vanneste, Janet Yu, Deidre Cornish, Heather McBrydie

Abstract: Bacterial canker of kiwifruit (Actinidia spp.), caused by Pseudomonas syringae pv. actinidiae (Psa, Biovar 3), seriously affected the New Zealand kiwifruit industry and requires new disease control products. Yeast isolates were screened for suppression of Psa and an effective isolate (YBCA5), identified as Aureobasidium pullulans (de Bary) G. Arnaud, was formulated and evaluated in field trials (2014 to 2017) and mode of action studies. Psa leaf spotting and bud browning were significantly reduced in the field trials across four years of study. Priming of plant host defence genes, leaf surface colonisation and altered pH are suggested as possible modes of action. Commercialisation of a product is progressing.

164-166

5.00 €

 

Aureobasidium pullulans strain Ach1-1 in biocontrol of postharvest diseases of apples: 15 crucial years of research before starting commercial development
Hanène Badri, E. Hassan Achbani, M. Haissam Jijakli

Extended abstract

167-168

0.00 €

 

Biocontrol products: From lab testing to product development
Javier Nacher Ibáñez

Abstract only

169

0.00 €

 

PhylloLux©, an alternative technology to synthetic fungicides for controlling major diseases of strawberries
Wojciech J. Janisiewicz, F. Takeda

Extended abstract

170-171

0.00 €

 

Licorice leaf extract as plant protection agent against tomato late blight – on the way from lab to field
Annegret Schmitt, Emilia Markellou, Athanasia Mandoulaki, Ina Kleeberg, Stavroula Konstantinidou-Doltsinis

Abstract: Licorice (Glycyrrhiza glabra) leaf extract was tested in the laboratory, on potted plants and under semi-commercial greenhouse and field conditions for control of late blight of tomato (Phytophthora infestans). The leaf extract at 2.5% (w/v) was reducing tomato late blight by 100% in detached-leaf assays. The leaf extract had no curative effect. Protective treatments of potted tomato plants with 1% (w/v) extract concentration reduced late blight to the same degree as the fungicide Fosethyl-Al. Under semi-commercial greenhouse conditions, licorice leaf extract (5% w/v) strongly reduced the disease severity of late blight compared to the control. In a small-scale field trial, licorice leaf extract (5% (w/v)) reduced disease severity of late blight on the last evaluation date by ca. 64%. In a large-scale field trial, the extract (5% (w/v)) reduced diseases severity and disease incidence per plant by 76% and 57%, compared to the control, respectively. Approx. 50% protection was still achieved in treated plots, at the time when control plots were completely destroyed by late blight.

172-174

5.00 €

 

Resilience, production and formulation issues in the production of biocontrol agents for disease control
Naresh Magan

Abstract only

176

0.00 €

 

Polyextremotolerant yeasts Aureobasidium spp.: antagonistic activity against phytopathogenic fungi
Janja Zajc, Cene Gostinčar, Maja Ravnikar, Nina Gunde-Cimerman

Extended abstract

177-178

0.00 €

 

Biocontrol products – current regulatory situation
Agata Jakubowska, Adi Cornelese, Gianpiero Gueli-Alletti, Inka Steingaß, Sabine Asser-Kaiser, Jacqueline Süß, Rüdiger Hauschild

Abstract only

179

0.00 €

 

Specific monitoring and quantification of the biological control agent Lactobacillus plantarum PM411 by viability qPCR
Núria Daranas, Anna Bonaterra, Jesús Francés, Jordi Cabrefiga, Emilio Montesinos, Esther Badosa

Abstract only

180

0.00 €

 

Can sclerotium size of Sclerotinia sclerotiorum be used as a predictor of susceptibility to Coniothyrium minitans?
Philippe C. Nicot, Christine Roy, Magali Duffaud, François Villeneuve, Marc Bardin

Abstract: In previous work, we observed wide levels of diversity among strains of Sclerotinia sclerotiorum in the susceptibility of their sclerotia to colonization by the mycoparasite Coniothyrium minitans. Here, we investigated a possible relationship between the level of in vitro susceptibility of the strains and the morphological traits of their sclerotia, with the aim of providing a simple predictive tool for field assessment. We focused on the average thickness of whole sclerotia and on that of the melanised cortical tissue that the mycoparasite needs to penetrate to colonize the medullar tissue. Significant differences were found among strains, with ranges of 0.80-1.72 mm and 51-84 μm, respectively, for sclerotium size (P < 0.001) and for cortex thickness (P = 0.05). However, sclerotium size was not correlated to any of the susceptibility indices examined for the strains (P values > 0.26; R² < 0.06). Cortex thickness was significantly correlated (P = 0.019; R² = 0.32) to the average frequency of detection of C. minitans in inoculated sclerotia but not to other susceptibility indices. These results suggest that other factors (possibly related to the biochemical composition of the tissues) play a determinant role in susceptibility of sclerotia to C. minitans. As a high level of intra-strain variability was observed (both in terms of morphological traits and susceptibility of sclerotia), work is under way to characterize individually larger numbers of sclerotia for selected strains.

181-186

5.00 €

 

Monitoring the dispersion and persistence of Penicillium frequentans Pf909 to field and postharvest conditions to control brown rot
Marta Balsells-Llauradó, Laura Vilanova, Neus Teixidó, Josep Usall, Belén Guijarro, Antonieta De Cal, Rosario Torres

Abstract only

187

0.00 €

 

Persistence of Bacillus amyloliquefaciens CPA-8-formulated products on nectarines and peaches after exposure to unfavourable environmental conditions
Amparo Gotor-Vila, J. Usall, R. Torres, N. Teixidó

Extended abstract

188-189

0.00 €

 

Resilience of different formulations of the biocontrol agent Candida sake CPA-1 on grapes under climate change conditions
Anna Carbó, Rosario Torres, Neus Teixidó, Josep Usall, Angel Medina, Naresh Magan

Abstract only

190

0.00 €

 

Biocontrol as a key component to manage brown rot diseases on cherry
Sophia Grannell

Abstract only

191

0.00 €

 

Water stress tolerance and transcriptional response in biological control strains of Lactobacillus plantarum from plant environments
Núria Daranas, Esther Badosa, Jesús Francés, Emilio Montesinos, Anna Bonaterra

Abstract only

192

0.00 €

 

First steps in the identification of arthropods associated to potato and corn orchards in organic farming in North Spain
Roberto Ruiz de Arcaute, S. Martínez, S. González-Cavero, R. Vercher, A. Ortiz

Abstract only

193

0.00 €

 

Osmotic tolerance response induced on survival ability at antagonist Yarrowia lipolytica M18/3B on apple fruit
Mónica Fernanda Burgos Zazueta, Ramón Álvar Martínez Peniche, Lourdes Soto Muñoz

Abstract: The population dynamics and antagonist activity of the yeast Yarrowia lipolytica M18/3B grown in basal medium NYDB modified a water activity (ɑw) of 0.97 with different solutes; glucose, glycerol, and NaCl were evaluated in apples. The results show that the highest development on fruit, was with the yeast grown in media modified with glycerol, followed by glucose and NaCl. Regarding, the antagonist activity against Penicillium expansum there were no significant differences between the glucose and glycerol treatments with respect to the basal medium.

194-196

5.00 €

 

Stability and biocontrol ability of Ralstonia solanacearum lytic bacteriophages in different environmental conditions
Belén Álvarez, Beatriz Martín, Alejandro Rodríguez, Begonya Vicedo, Elena G. Biosca

Extended abstract

197-198

0.00 €

 

Challenges in developing microbial seed treatments
Gertjan van Westen

Abstract only

200

0.00 €

 

Improving biological control using microbiota: the case of apple for postharvest diseases
Abdoul Razack Sare, M. Haissam Jijakli, Sebastien Massart

Abstract only

201

0.00 €

 

Efficacy of three biocontrol agents applied individually and in combination in the control of Neofusicoccum parvum, a fungal pathogen involved in grapevine trunk disease
Amira Yacoub, Rana Haidar, Jonathan Gerbore, Marie-Cécile Dufour, Marie-France Corio-Costet, Patrice Rey

Abstract only

202-203

0.00 €

 

Recent advances in the analysis of the olive fungal microbiome
Ahmed Abdelfattah, Santa Olga Cacciola, David Ruano Rosa, Giulia Li Destri Nicosia, Imen Belgacem, Saveria Mosca, Giovanni E. Agosteo, Gaetano Magnano di San Lio, Leonardo Schena

Extended abstract

204-206

0.00 €

 

Microbial community composition in take-all suppressive soils
Paola Durán, Gonzalo Tortella, Sharon Viscardi, María de la Luz Mora, María José Pozo

Abstract only

207

0.00 €

 

Fermented foods as source for novel biocontrol microbial consortia
Michael Wisniewski, Antonio Biasi, Yelka Zhimo, Mariana Gondo, Silvana Vero, Samir Droby

Abstract only

208

0.00 €

 

Better together! Insights from the development of a biological control product based on combination of different microorganisms – a way to success?
Robert Czajkowski, Dorota M. Krzyzanowska, Tomasz Maciag, Joanna Siwinska, Sylwia Jafra

Abstract only

209-210

0.00 €

 

Integrated approach to control soil-borne pests in organic strawberry plantations
Eligio Malusa, Małgorzata Tartanus, Loredana Canfora, Flavia Pinzari, Anna Benedetti, Cezary Tkaczuk and Barbara Helena Łabonowska

Abstract only

211

0.00 €

 

Design and development of a biological fungicide composed of a consortium of Bacillus subtilis
José Luis Velasco Silva, Ileana Rubio Velázquez, Ricardo Olivares Moreno, Víctor Alfonso Sáenz Álvaro

Abstract only

212

0.00 €

 

Preventing post-harvest losses in sugar beet: Biological control consortia with antagonistic and VOCs producing potential
Peter Kusstatscher, Tomislav Cernava, Karsten Harms, Johann Maier, Herbert Eigner, Daniel Treffer, Gabriele Berg, Christin Zachow

Abstract only

213-214

0.00 €

 

Biological control of root-knot nematode Meloidogyne incognita infesting eggplant by the nematode-trapping fungus Dactylaria brochopaga and the nematode egg parasitic fungus Verticillium chlamydosporium under field conditions
E. M. A. Noweer

Abstract only

215

0.00 €

 
Add this product to your basket