Abstract: One of the most important requirements for high efficacy of the bait sprays against olive fruit fly Bactrocera oleae (Rossi) (Diptera: Tephritidae) is the prolonged residual activity of the baits. The currently used lasts only 5-6 days, which is not enough to cover the practically continuous emergence of adults. The finding of long-life attractant solutions constitutes the main objective of an ongoing research effort. In the present study laboratory choice experiment was conducted to determine the response of female olive fruit fly to various stimuli (attractant solutions used in bait sprays, olive fruit, food, unsprayed shoot) by using a time-lapse video system. Our results have shown the superiority of the Success at the very dense dose (8.3%) compare to the other tested attracted solutions in all the parameters studied: percentage of adult females attracted, shorter period of time until it touched the sprayed surface, shorter residence time of the insect on the sprayed shoot, and exclusively first preference of the females on the sprayed shoot compare to other stimuli. Automated observation using long-term uninterrupted time-lapse video is particularly suitable for measuring insect behaviors that the human observer is unable to accurately estimate.