Effect of top spray drying and freeze drying on the photostability and insecticidal activity of a Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV 003) formulation

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Abstract: The nucleopolyhedroviruses are considered as an effective biopesticide against the fallarmyworm Spodoptera frugiperda. Top spray and freeze drying methods were used to prepare awettable powder formulation based on nucleopolyhedrovirus of S. frugiperda (SfMNPV 003) andits photostability and virulence were assessed. Top spray drying method was more efficient forremoving moisture content than freeze drying. No obvious differences in the insecticidalactivities were observed for both drying methods although a higher photostability (88.54%) wasobserved in the formulation prepared with top spray drying method compared to freeze drying(77.77%) and unformulated virus (15.62%) after 6 hours of UV radiation exposure. Top spraydrying method was selected as the most favorable process for being implemented in amanufacture process.

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