Abstract: To determine if elicitors produced by biocontrol F. sambucinum (isolate FS-94) andP. fluorescens (strain 197) were able to induce spring wheat resistance against multiplepathogens, intracellular and extracellular elicitors of FS-94 as well as MF3-protein of 197 wereused for treatment. The treatment of wheat seeds was conducted before their artificial inoculationwith F. culmorum or seeds naturally infected with agents of root rots. Intracellular elicitors of FS-94 induced resistance in spring wheat against multiple fungi belonging to pathogenic root rotcomplex in Non-Chernozem zone of Russia (F. culmorum, F. avenaceum, F. oxysporum,F. sporotrichioides, F. gibbosum, B. sorokiniana). These elicitors decreased the incidence andseverity of the diseases, reduced pathogen infectivity and suppressed sporulation of all tested rootrot agents, except F. avenaceum. Extracellular elicitors of FS-94 and MF3 induced no wheatresistance to Fusaria. The both types of FS-94 elicitors prevented Stagonospora nodorumdevelopment on wheat leaves, while MF3 was active against St. nodorum only in combinationwith chitosan.