Elicitors of Leptosphaeria maculans inducing resistance to blackleg in oilseed rape

Abstract: Compounds produced by L. maculans into various cultivation media were tested for their ability to induce defence gene expression by means of RT-qPCR and SAR by inoculation test. After removing toxins and other low molecular metabolites by dialysis, the medium was concentrated and subsequently subjected to fractionation by ionex chromatography. Separated fractions inducing PR1 expression were digested by trypsin. Tryptic hydrolysates lost their PR1-inducing activity, which indicates a protein nature of the efficient elicitors.