Abstract: Due to the reduction of peat in ornamental substrates, great attention is focusing onsetting up new and innovative substrates for this market. In the present work, Trichoderma spp.isolates were selected for endophytism and plant growth promotion in Limonium sinuatum,Cupressus sempervirens and Camelia sinensis. Ten isolates (out of 162) for Limonium, 9 (out of162) for Cupressus and 8 (out of 202) for Camelia resulted endophytic in roots. From asuccessive round of inoculation three Trichoderma isolates, among which T2046 was in commonfor all the tested species, confirmed the best endophytic performance and improved growth. Inaddition, the antagonistic activity of selected strains against fungal plant pathogens such asBotrytis cinerea, Colletotrichum gloeosporioides and Rhizoctonia solani have been evaluated.The three Trichoderma isolates, and first of all T2046, could be taken into account as inoculantsfor innovative substrates for ornamental plants.