Abstract: This study describes the resistance of 300 accessions of various species of Brassica to clubroot disease caused by Plasmodiophora brassicae. The examined lines originated from the Center for Genetic Resources for Food and Agriculture in the Netherlands (CGN) and Plant Gene Resources in Canada (CN). Studied plant materials included Brassica oleracea, B. rapa, B. napus and B. villosa. Six pathogen pathotypes, collected in Poland were used for the plant inoculations – P1, P1(+), P2, P3, P4 and P5. The experiments were carried in greenhouse conditions. In most cases the observed resistance was pathotype specific and only one Brassica form, three accessions of B. oleracea, were resistant to all the examined pathotypes of P. brassicae, including the P1B pathotype, which is the most damaging to Brassica plants in Poland. Resistance to clubroot was rare and mostly pathotype-specific.