Abstract: The aim of this study was to identify the sources of genetic resistance to clubroot and to obtain the interspecific hybrids with increased level of resistance. The plant material consisted of five high yielding cultivars (Anderson, Arsenal, Andromeda, Californium and Monolit) of Brassica napus and selected Brassica rapa genotypes with increased resistance to Plasmodiophora brassicae. All interspecific hybridizations were performed with the application of an in vitro embryo culture. The effectiveness of interspecific crosses varied widely depending on the genotype used as a pollinator. The lowest efficiency was observed in crosses between B. napus cv. Arsenal and B. rapa var. rapa.