Studies on the storage of formulated Metarhizium anisopliae conidia: effect oftemperature on conidia germination and virulence against Tenebrio larvae


Abstract: The entomopathogenic fungus Metarhizium anisopliae, applied under field conditions,needs to be formulated to reduce the damage that can be caused, because of (i) UV-radiation, (ii)high temperatures and (iii) contact with water. The aim of our work was to study the longevityand virulence of conidia of M. anisopliae, mixed with six different anorganic fillers (Seramis®,CN- Blähton®, Liadrain®, Clever Cat Litter®, Green Clay®, Tixosil™). The formulated andunformulated conidia were stored over several time intervals (1 week, 1 month, 3 and 6 months)at different temperatures (25°C, 4°C, -20°C).No remarkable differences were seen between formulated and unformulated conidia storedat 4°C and -20°C. Formulated conidia stored at 25°C for six months lost hundred percent of theirvitality in comparison to unformulated conidia and those, which were stored at coldertemperatures. All four expanded clay materials and Green Clay® were well suited for the storageof a virulent M. anisopliae at 4°C and -20°C. Neither the production methods nor the differentexpanded clay materials had a negative effect on the virulence of BIPESCO 5 conidia.

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