Abstract: Pseudomonas spp. (Ps) infection in wheat induces systemic immunity against Xanthomonas translucens pv. cerealis (Xtc). RNA-seq analyses revealed that Ps potentiates defence by the accumulation of ERF and WRKY transcription factors (TFs). Additionally, Ps ‘primes’ a subset of genes regulated specifically when challenged by Xtc, including a Triticum aestivum Ps-primed (TaPsP) TF.