qPCR and NGS techniques for pathogen detection and monitoring of microbial communities in soil after application of broad spectrum fungal treatments in cucumber crop

Abstract: Nowadays, the study of the fungicides effects on non-target microorganisms has become important because of biodiversity losses. It was analysed the impact of two broad spectrum fungicides (fluopyram and penthiopyrad) on soil microorganisms. Bacterial and fungal communities were affected by fungicides two months after the end of the application indicating the persistence of fungicides in soil.